Differentiation Landscape
Landscape
All inferred binary fate choices were integrated with normal fate progression to generate a landscape of lineage differentiation with 56 trajectories and 32 types of regulated fate choices. This directed graph summarizes all possible trajectories a cell can take to differentiate into a specific fate and the corresponding regulatory switch genes that determine the differentiation path along the trajectories.
Nodes represent cell fates and arrows represent trajectories of differentiation. Black arrows represent trajectories observed during normal development; green arrows represent alternative trajectories observed due to gene perturbation. All detected alternative trajectories are labeled by number. Each open circle represents a decision point of cell fate choice. Regulators of the fate choice between default and alternative trajectories are shown below.
Trajectory | Alternative Path | Normal Path | Genes |
---|---|---|---|
1 | P0-to-ABp | P0-to-AB | cdc-25.1,cls-2,div-1,puf-3,rfc-3,zyg-11 |
2 | P0-to-C | P0-to-AB | mex-3 |
3 | P0-to-EMS | P0-to-AB | mbk-2, mex-5, par-3, zyg-11 |
4 | P0-to-P2 | P0-to-P1 | cdc-25.1, div-1 |
5 | AB-to-ABa | AB-to-ABp | apx-1,atx-2,glp-1,lag-1,par-2,par-6,pie-1,pkc-3,pos-1,rga-3,sel-8 |
6 | AB-to-ABp | AB-to-ABa | F23F1.5,ceh-24,csn-4,cul-2,npp-4,ran-4,wwp-1 |
7 | P1-to-C | P1-to-EMS | F44B9.8,Y53F4B.22,atx-2,cdc-37,mbk-2,oma-1/2,skn-1,tsr-1 |
8 | P1-to-EMS | P1-to-P2 | arp-11,par-2,par-6,pie-1,pkc-3 |
9 | ABa-to-ABal | ABa-to-ABar | atx-2,camt-1,ceh-24,cpl-1,cul-1,cul-3,gld-2,gsk-3,hmp-2,let-502,lin-23,mei-1,mom-2,mom-5,oma-1/2,par-2,par-3,pos-1,ppfr-4,puf-3,rga-3,skr-1/2,tag-256,tat-5,uba-1 |
10 | ABal-to-ABarp | ABal-to-ABala | cul-3 |
11 | ABal-to-ABarp | ABal-to-ABalp | cdc-37,glp-1,lag-1,oma-1/2,par-6,pkc-3,ran-4,sel-8,skn-1 |
12 | ABal-to-ABara | ABal-to-ABalp | T11G6.8, rad-51 |
13 | ABal-to-ABalp | ABal-to-ABala | par-3 |
14 | ABar-to-ABala | ABar-to-ABara | glp-1, lag-1, par-6, sel-8, skn-1 |
15 | ABal-to-ABara | ABal-to-ABala | arp-11,atx-2,cdc-37,ceh-24,chaf-2,chk-1,cpl-1,cul-1,dlc-1,dli-1,gld-2,gsk-3,hda-1,let-502,nud-1,par-3,rba-1,rga-3,skr-1/2,tat-5,ubc-12,vps-4 |
16 | ABar-to-ABara | ABar-to-ABarp | gpb-1 |
17 | ABar-to-ABalp | ABar-to-ABarp | ceh-24, gpb-1, ubc-12 |
18 | ABpl-to-ABplp | ABpl-to-ABpla | drh-3 |
19 | ABpl-to-ABpra | ABpl-to-ABplp | cul-3 |
20 | ABpr-to-ABpla | ABpr-to-ABpra | camt-1,cul-3,gld-2,let-502,lin-23,mei-1,mom-2,mom-5,oma-1/2,ppfr-4,rab-7,ran-4,rga-3,skn-1,skr-1/2,tag-256,tat-5 |
21 | ABpl-to-ABpra | ABpl-to-ABpla | F35F11.1,ceh-24,cul-3,glp-1,gsk-3,lag-1,nmy-2,npp-4,pad-1,pop-1,rab-5,rga-3,ruvb-1,suf-1,wwp-1,zyg-12 |
22 | ABpr-to-ABpra | ABpr-to-ABprp | cul-3 |
23 | EMS-to-EMS | EMS-to-MS | cul-1, lin-23, skr-1/2, zyg-11 |
24 | P2-to-EMS | P2-to-C | cul-1, gsk-3, lin-23, skr-1/2 |
25 | P2-to-C | P2-to-P3 | cdc-37 |
26 | P2-to-MS | P2-to-C | gei-17 |
27 | EMS-to-E | EMS-to-MS | pop-1 |
28 | EMS-to-MS | EMS-to-E | gld-2,gsk-3,mom-2,par-2,par-6,pkc-3 |
29 | C-to-C | C-to-Cx | cul-1, skr-1/2 |
30 | P3-to-P3 | P3-to-P4 | cul-1, lin-23, skr-1/2 |
31 | P3-to-P4 | P3-to-D | mex-3 |
32 | P3-to-D | P3-to-P4 | gld-2, mex-5, src-1 |